More info
Assay Range | 938-60,000 pg/mL |
Sensitivity | 900 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human C4BP standard: 1 vial
2. One 96-well plate coated with Human C4BP Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human C4BP Ab (50x): 140 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Complement component C4b-binding protein (C4BP) is a glycoprotein with a molecular weight of 570 kDa. it is present in plasma in various isoforms with different compositions. The major form of C4BP is composed of seven identical 70-kDa alpha chains, each containing a binding site for the complement protein C4b, and a unique 45-kDa beta chain which contains a binding site for the vitamin K-dependent protein S. C4BP regulates the complement system by accelerating the decay of the complement component C3 convertase and by acting as a cofactor to the serine protease factor I in the degradation of C4b. C4BP was overexpressed in the synovial membranes of patients with rheumatoid arthritis . It was detected in amyloid-beta plaques and on apoptotic cells in Alzheimer's disease brain. C4BP could directly bind to ovarian tumor cell lines and was associated with non-small cell lung cancer.