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Human EphB6 ELISA Kit

Catalog #: NS-E10191

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Human EphB6 ELISA Kit

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Data sheet

Assay Range	9.4 - 600 pg/ml

More info

Sensitivity

5 pg/mL

Size

96T

Storage

Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC

Assay Principle

Sandwich ELISA

Sample volume

100 µL final volume, dilution factor varies on samples.

Detection Method

Chromogenic

Kit Components

1. Recombinant human EphB6 standard: 0.6 ng/vial - 2.

2. One 96-well plate precoated with anti-human EphB6 Ab

3. Sample diluent buffer: 12 mL x 1

4. HRP conjugated anti-Human EphB6 Ab: 1 vial, dilution 1:1000.

5. Detection Antibody diluent buffer: 12 mL x 1

6. 20 x Wash Solution: 25 ml x 1.

7. TMB color developing agent A: 6 ml.

8. TMB color developing agent B: 6 ml.

9. TMB stop solution: 6ml.

Background

EPHB6 is a kinase-defective receptor for members of the ephrin-B family that function as receptors for ephrin-B family proteins. It is a transmembrane protein which binds to ephrin-B1 and ephrin-B2 and modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. It also inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2. EPHB6 is expressed in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney. Expression of EPHB6 is downregulated during tumor progression, suggesting that the protein may suppress tumor invasion and metastasis.

Standard Curve

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Protocol

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