Data sheet
| Assay Range | 9.4 - 600 pg/ml |
More info
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human EphB6 standard: 0.6 ng/vial - 2.
2. One 96-well plate precoated with anti-human EphB6 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human EphB6 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
EPHB6 is a kinase-defective receptor for members of the ephrin-B family that function as receptors for ephrin-B family proteins. It is a transmembrane protein which binds to ephrin-B1 and ephrin-B2 and modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. It also inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2. EPHB6 is expressed in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney. Expression of EPHB6 is downregulated during tumor progression, suggesting that the protein may suppress tumor invasion and metastasis.
