Data sheet
| Assay Range | 31.2--2000 pg/ml |
More info
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human SerpinA1 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human SerpinA1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human SerpinA1 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
SerpinA1, also known as Alpha-1 antitrypsin (A1AT), is a prototype member of the Serpin superfamily of the serine protease inhibitors. SerpinA1 is synthesized in hepatocytes and secreted as the abundant proteinase inhibitor in the circulation. SerpinA1 primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. It irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The major physiological function of SerpinA1 is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). SerpinA1 deficiency in circulation is associated with emphysema, liver disease and Alpha-1-antitrypsin deficiency (A1ATD).
