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Assay Range | 78.1-5,000 pg/mL |
Sensitivity | 78.1 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human C2 standard: 2 vials
2. One 96-well plate coated with Human C2 Ab
3. Diluent buffer: 30 ml - 1
4. HRP- antibody Conjugate (100x): 1 vial
5. TMB developing agent: 11 mL x2
6. Stop solution: 6 mL x1
7. Washing solution (20x): 25 mL x1
Background
Complement C2 is a 732 amino acid multi-domain serum protease. The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1 complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. After activation by C1, the single chain form of C2 becomes two chains, C2A and C2B. C2A consists of a vWF domain and a serine protease domain. C2B is characterized by 3 Sushi (SCR) domains. C2A, then combines with complement factor 4B to generate the C3 or C5 convertase. It is reported that polymorphism in C2 gene is associated with progression to advanced age- related macular degeneration with visual loss.