Taq DNA Polymerase Recombinant ( TaqDNA )

Catalog # PT_42186

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$201.00
DescriptionTaq DNA Polymerase(a) is a thermostable enzyme of approximately 94 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization
Product Size: 1,000U
Quantity

Data sheet

Formulation
Taq DNA Polymerase solution in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Description
Taq DNA Polymerase(a) is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides in
Expression host
Recombinant e.coli contains Thermus aquaticus polymerase gene.
Synonyms
DNA polymerase I thermostable, EC 2.7.7.7, Taq polymerase 1.
Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200um each of dATP,
Storage Conditions
Stable for 5 days at 10°C, for longer period of time store at -20°C.
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