DNA Topoisomerase-I 70kDa Recombinant Human ( TOP1 70kDa Human )

Catalog # PT_72030

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$201.00
DescriptionRecombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 69,199 Dalton (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag.SourceSf9 insect cells.FormulationTOP1 70kDa is supplied in 16mM HEPES
Product Size: 5ug
Quantity

Data sheet

Formulation
TOP1 70kDa is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.
Purity
Greater than 80% as determined by SDS-PAGE.
Applications
Western blot with Scleroderma patient sera or monoclonal anti-hexa-His-tag antibody.
Description
Recombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 69,199 Dalton (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag.
Protein Background
TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in
Expression host
Sf9 insect cells.
Synonyms
DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Coating concentration
0.5-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
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