Selenocysteinyl-tRNA ( Sec ) synthase Human Recombinant ( SEPSECS Human )

Data sheet

Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Formulation

The SEPSECS protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 100mM NaCl and 10% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

SEPSECS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 521 amino acids (1-501a.a.) and having a molecular mass of 57.9kDa.SEPSECS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic te

Protein Background

SEPSECS catalyzes the last step of sec synthesis by way of converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor. Furthermore, SEPSECS protein is considered a specific marker of autoimmune hepatitis.

Expression host

Escherichia Coli.

Synonyms

SLA, SLA/LP autoantigen, SLA-p35, Selenocysteinyl-tRNA(Sec) synthase, Selenocysteine synthase, Liver-pancreas antigen, Soluble liver antigen, Sec synthase, UGA suppressor tRNA-associated protein.

Reagent Appearance

Sterile Filtered clear solution.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLLLEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRP KAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEGDELRT DLKAVEAKVQ EL