RIPA Buffer

Product Description

Extraction of cellular proteins requires efficient cell lysis and protein solubilization, while avoiding protein degradation and/or interference with protein immunoreactivity and biological activity. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured cells. It has long been a widely used lysis and wash buffer for small-scale affinity pull-down applications, such as immunoprecipitation, since most antibodies and protein antigens are not adversely affected by the components of this buffer. 1 In addition, RIPA Buffer minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. RIPA Buffer is supplied as a ready to use solution that requires no preparation. Protease and phosphatase inhibitors may be added to the lysis buffer as needed. One ml of the RIPA Buffer is sufficient to lyse cells from one 100 mm culture dish of most adherent mammalian cell lines.

Components

RIPA Buffer (Cat# NBP-115) – 50 mM Tris-HCl, pH 7.6, with 150 mM sodium chloride, 1.0% Igepal CA-630 (NP-40), 0.5% sodium deoxycholate, and 0.1% sodium dodecyl sulfate

For research use only. Not for use in diagnostic procedures.