Bovine insulin-like growth factor 1 IGF-1 ELISA Kit

Product name:  Bovine insulin-like growth factor 1 IGF-1 ELISA Kit

Catalog No. BG-BVN21180

Assay Principle:  Sandwich ELISA

Size: 96T

Range: 31.25 pg/mL -2000 pg/mL

Sensitivity: Less than 15 pg/mL

Storage: Store at 2-8â°C.

Expiration: Six months.

Application: cell culture, supernatant, serum, plasma and other suitable sample solution.

Kit Components:

1. Bovine IGF-1 standard: 50 ng/mL - 1.

2. One 96-well plate precoated with anti-Bovine IGF-1antibody.

3. Sample diluent buffer: 10 mL - 3

4. Biotinylated anti-Bovine IGF-1 antibody: 60 µL, dilution 1:200.

5. Streptavidin-HRP: 60 µL, dilution 1:200.

6. Chromogenic Solution A: 6 ml

7. Chromogenic Solution B: 6 ml

8. TMB stop solution: 6 ml.

9. 20 - Wash Solution: 25 ml

Background

Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. IGF-1 production is stimulated by growth hormone (GH) and can be retarded by under nutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signalling pathway post GH receptor. 

 PUBLICATION:

1. D. Đuričić1 et al. Effects of dietary clinoptilolite on reproductive performance, serum progesterone and insulin-like growth factor-1 concentrations in dairy cows during pregnancy and lactation. Polish Journal of Veterinary Sciences Vol. 23, No. 1 (2020), 69–75