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Bovine Beta Casomorphin, β-CM ELISA Kit | Bovine Beta Casomorphin, Beta-CM ELISA Kit
BG-BVN11953
Data sheet
| Assay time | 2.5 hours |
| Validity | 6 months |
| Storage | See manual |
| Background | Caseins and whey proteins from bovine milk represent a source of biologically active peptides which may exert ‘‘pharmacological” activities. Milk-derived bio-peptides are encrypted in an inactive form within the sequences of bovine milk proteins from which they are potentially released during fermentation or in vivo or in vitro digestion. In recent literature, the bioactivity of milk-derived peptides has been extensively reviewed and several activities (opioid, blood pressure-lowering, antimicrobial and immunomodulatory) have been reported b-casomorphins (BCMs)are exogenous opioid peptides deriving from proteolysis of b-casein (b-CN) and they were originally isolated from an enzymatic digest of casein. Amongst BCMs, b-casomorphin-7 (BCM7) and b-casomorphin-5 (BCM5) represent fragments f60– 66 and f60–64 of bovine b-CN, respectively. The release of BCM during hydrolysis of bovine b-CN seems to be dependent on the presence of some variants of this protein, such as A1 and B. These variants have histidine at position 67, whereas proline is present in variant A2. This amino acidic substitution likely prevents the hydrolysis of the Ile66–Pro67 bond during proteolysis thus hindering the release of BCM7 from b-CN A2. Some ecological studies suggested that BCM7 or related peptides may increase the risk for the development of some human diseases such as juvenile diabetes type I, ischaemic hearth disease, autism and schizophrenia. |
More info
Bovine Beta Casomorphin, β-CM ELISA Kit
For research use only. Not for use in diagnostic procedures
Assay Range: 370 - 90,000 pg/ml
Sensitivity: 100 pg/ml
Precision: Intra-Assay, <8%; Inter-Assay, < 12%