View larger
Bovine Matrix Metalloproteinase 9 MMP-9 ELISA Kit
BG-BVN11611
Bovine Matrix Metalloproteinase 9 ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction
More info
Product name: Bovine Matrix Metalloproteinase 9, MMP9 ELISA Kit
Catalog No. BG-BVN11611
Assay Principle: Sandwich ELISA
Size: 96T
Range: 15.6pg/mL -1000 pg/mL
Sensitivity: 10 pg/mL
Storage: Store at 2-8â°C.
Expiration: Six months.
Application: cell culture, supernatant, serum, plasma and other suitable sample solution.
Kit Components:
1. Bovine MMP-9 standard: 10 ng/mL - 1.
2. One 96-well plate precoated with anti-Bovine MMP-9 antibody.
3. Sample diluent buffer: 10 mL - 1
4. Biotinylated anti-Bovine MMP-9 antibody: 60 µL, dilution 1:200.
5. Streptavidin-HRP: 60 µL, dilution 1:200.
6. Chromogenic Solution A: 6 ml
7. Chromogenic Solution B: 6 ml
8. TMB stop solution: 6 ml.
9. 20 - Wash Solution: 25 ml
Background
Matrix metallopeptidase 9 (MMP-9), also known as Type IV collagenase and gelatinase B, is an enzyme that belongs to the MMP family. MMPs such as MMP9 can be involved in the development of several human malignancies, as degradation of collagen IV in basement membrane and extracellular matrix facilitates tumor progression, including invasion, metastasis, growth and angiogenesis.