Bovine tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

Product name:  Bovine tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

Catalog No. BG-BVN20004

Assay Principle:  Sandwich ELISA

Size: 96T

Range: 156 pg/mL -10,000 pg/mL

Sensitivity: Less than 5 pg/mL

Storage: Store at 2-8⁰C.

Expiration: Six months.

Application: cell culture, supernatant, serum or plasma.

Kit Components:

1. Lyophilized recombinant Bovine TIMP-1standard: 10 ng/tube-2.

2. One 96-well plate precoated with anti-Bovine TIMP-1 antibody.

3. Sample diluent buffer: 12 mL - 1

4. Biotinylated anti-Bovine TIMP-1 antibody: 130 µL, dilution 1:100.

5. Antibody diluent buffer: 12 ml.

6. Streptavidin-HRP: 130 µL, dilution 1:100.

7. Streptavidin-HRP diluent buffer: 12 ml.

8. TMB color developing agent: 10 ml.

9. TMB stop solution: 10 ml.

10. 20 - Wash Solution: 25 ml

Background

TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Over expression of TIMP-1 correlates with aggressive clinical behavior of a spectrum of tumors.