Human CXCL6/GCP-2 ELISA Kit
Catalog # NR-E10147
$692.50
For research use only. Not for diagnostic or therapeutic use, or for human or veterinary use. Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.
Assay Range | 31.2--2,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant human CXCL6 standard: 2 vials.
2. One 96-well plate precoated with anti-human CXCL6 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Chemokine (C-X-C motif) ligand 6 (CXCL6), also known as Chemokine alpha 3 (CKA-3), granulocyte chemotactic protein 2 (GCP-2), or small-inducible cytokine B6, is a small cytokine belonging to the CXC chemokine family. Human CXCL6 shares 60% sequence identity with mouse CXCL6. Three N-terminally truncated isoforms of human CXCL6 have been isolated, and show no significant variation in activity from the mature protein, while N-terminally truncated forms of mouse CXCL6 are more active than the mature form, and are more active than human CXCL6. CXC chemokine receptor 1 (CXCR1) and CXCR2, expressed on neutrophils, but not on T- or B-lymphocytes, are the receptors for CXCL6 binding and activation. CXCL6 promotes neutrophil migration and activation both in vitro and in vivo. CXCL6 may contribute to sepsis-associated tissue damage, hypersensitivity reactions, autoimmune conditions, and tumor invasion.
New