Proliferating Cell Antigen Human Recombinant, Sf9 ( PCNA Human, Sf9 )

Data sheet

Formulation

Recombinant Human PCNA is supplied in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol.

Purity

Greater than 95% as determined by SDS-PAGE.

Applications

Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

Description

PCNA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 35 kDa. PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Protein Background

Proliferating cell nuclear antigen (PCNA), a protein of 30 kDa molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA rep

Expression host

Sf9 insect cells.

Synonyms

Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.

Reagent Appearance

Sterile Filtered colorless solution.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Immunological functions

1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera.

Coating concentration

0.5-1.1 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.