Data sheet
| Size | 50 µg, 313 pmol |
| Application | Recombinant dCas9 (D10A & H840A) protein is suitable for use in imaging of genomic loci in living cells and fixed cells as well as for specific sequence pull-down. |
| Background | The functions of CRISPR and CRISPR-associated (Cas) genes are essential in adaptive immunity in some bacteria and archaea. CRISPR uses a Cas9 protein to recognize DNA sequences, with target specificity solely determined by a small guide (sg) RNA and a protospacer adjacent motif (PAM). Upon binding to target DNA, the Cas9-sgRNA complex generates a DNA double-stranded break. Based on this RNA- guided nuclease activity, CRISPR has been showed to be a powerful tool in editing the genomes of a broad range of organisms. Furthermore, a repurposed, nuclease-deactivated Cas9 (dCas9) protein has been used to regulate endogenous gene expression and labeling of genomic loci in living and fixed cells. dCas9-SBP was used for specific sequence enrichment from samples and pull-down in CHIP assay. |
| Formulation | Recombinant dCas9 (D10A & H840A) protein expressed in E. coli supplied in a buffer of 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol. |
| Host/Source | E. coli |
| Sequence | Mutated CRISPR-associated endonuclease Cas9 (amino acids 1 to 1368) with D10A & H840A (Uniprot: Q99ZW2 for Cas9) |
| Amino Acids | D10A/H840A |
| Concentration | 1 µg/ µL, 6.26 µM |
| Physical appearance | Sterile filtered colorless solution. |
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