More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| SMAD family member 4 | Monoclonal | IgG | Mouse | Human | ICC, WB | Ascites |
Immunogen | ||||||
| Recombinant human Smad4(DPC4). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SMAD4 |
Protein | Mothers against decapentaplegic homolog 4 |
Uniprot ID | Human(Q13485), Mouse(P97471), Rat(O70437) |
Function | In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R- SMAD. PDPK1 prevents its nuclear translocation in response to TGF- beta. |
Sequence Similarities | Belongs to the dwarfin/SMAD family. |
Aliases | JIP|DPC4|MADH4|MYHRS|MAD homolog 4|MAD,mothers against decapentaplegic homolog 4(Drosophila)|SMAD, mothers against DPP homolog 4(Drosophila)|SMA- AND MAD-RELATED PROTEIN 4|DELETED IN PANCREATIC CARCINOMA 4|Deletion target in pancreatic carcinoma 4|SMAD family member 4 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 2-4μg/ml | Human | AssaySolutio's ECL kit |
| Immunocytochemistry | Suitable | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for ICC. *Blocking peptide can be purchased at $65. Contact us for more information
-antibody-(monoclonal)-ASA-B1732-1.jpg)
Anti-Smad4(DPC4) antibody (monoclonal), ASA-B1732, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
-antibody-(monoclonal)-ASA-B1732-2.jpg)