More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Rac1 (169-189aa FDEAIRAVLCPPPVKKRKRKC), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RAC1 |
Protein | Ras-related C3 botulinum toxin substrate 1 |
Uniprot ID | P63000 |
Function | Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. |
Tissue Specificity | Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. |
Sub-cellular localization | Cell membrane ; Lipid-anchor ; Cytoplasmic side . Melanosome. |
Sequence Similarities | Belongs to the small GTPase superfamily. Rho family. |
Aliases | Cell migration inducing gene 5 protein antibody|Cell migration-inducing gene 5 protein antibody|MGC111543 antibody|MIG5 antibody|Migration inducing gene 5 antibody|Migration inducing protein 5 antibody|p21 Rac1 antibody|p21-Rac1 antibody|Rac 1 antibody|RAC1 antibody|RAC1_HUMAN antibody|Ras like protein TC25 antibody|Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody|Ras-like protein TC25 antibody|Ras-related C3 botulinum toxin substrate 1 antibody|Rho family small GTP binding protein Rac1 antibody|TC 25 antibody|TC25 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- RAC1 antibody, ASA-B1604, Western blotting
All lanes: Anti RAC1 (ASA-B1604) at 0.5ug/ml
Lane 1: Rat Pancreas Tissue Lysate at 50ug
Lane 2: Mouse Intestine Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: SGC Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
All lanes: Anti RAC1 (ASA-B1604) at 0.5ug/ml
Lane 1: Rat Pancreas Tissue Lysate at 50ug
Lane 2: Mouse Intestine Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: SGC Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
