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8.iso.prostaglandin F2A 8IsoPGF2a ELISA Kit
BG-HUM11781
Human 8.Iso.Prostaglandin F2A ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction
Data sheet
| Assay time | 1 to 1.5 hours |
| Assay Range | 1.25–80 ng/ml |
| Sensitivity | 1.00 ng/mL |
| Expiration | 2-8 °C |
| Background | 8-hydroxy-2’-deoxyguanosine (8-OHdG) is a modified nucleoside base, which is the most commonly studied and detected by-product of DNA damage that is excreted in the urine upon DNA repair. Urinary 8-OHdG and its analogs, 8-hydroxyguanosine and 8-hydroxyguanine, are linked to many degenerative diseases. The association of ROS and the use of 8-OHdG as a biomarker of oxidative stress have been investigated in many diseases, including bladder and prostate cancer, cystic fibrosis, atopic dermatitis and rheumatoid arthritis. Parkinson’s disease, Alzheimer’s disease and Huntington’s disease are neurodegenerative diseases that are thought to be caused by exposure to neurotoxins in people with a genetic predisposition for these diseases. Oxidative stress is associated with the pathogenesis of these diseases and elevated levels of DNA damage have been measured in a wide range of neurological conditions. |
| Assay principle | The DNA Damage ELISA (enzyme-linked immunosorbent assay) is a fast and sensitive competitive immunoassay for the detection and quantitation of 8-hydroxy-2’-deoxyguanosine (8-OHdG) in urine, serum, and saliva samples. 8-OHdG has become a frequently used biomarker of oxidative DNA damage and oxidative stress. Measurement of urinary 8-OHdG may be useful as an indicator of oxidative damage. The DNA Damage ELISA uses an 8-OHdG monoclonal antibody to bind, in a competitive manner, 8-OHdG in the sample, standard or pre-bound to the wells of the 96-well immunoassay plate. Anti-8-OHdG bound to 8-OHdG in the sample or standard are washed away while those captured by the immobilized 8-OHdG are detected with a secondary antibody: HRP conjugate. The assay is developed with tetramethylbenzidine substrate and the absorbance is measured in a microplate reader at 450nm. The intensity of the yellow color is inversely proportional to the concentration of 8-OHdG. |
More info
Different lot may vary, please follow the instruction coming with the kit.