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Small Nuclear Ribonucleoprotein Polypeptide A Human Recombinant ( SNRPA1 Human )
Data sheet
| Formulation | SNRPA1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.2M NaCl,2mM DTT and 0.1mM PMSF. |
| Purity | Greater than 80% as determined by SDS-PAGE. |
| Description | SNRPA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 275 amino acids (1-255 a.a.) and having a molecular mass of 30.5kDa. SNRPA1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background | U2 small nuclear ribonucleoprotein A (SNRPA1), a 255 amino acid protein, is a component of the U2 snRNP which forms a complex with U2 snRNP B (U2B). U2 snRNP A and U2 snRNP B form together a complex which binds to the U2 snRNA hairpin IV. The configuration of the U2 snRNP A/U2 snRNP B dimer and the delicate variations of a few key residues regulate the snRNP-RNA-binding specificity. |
| Expression host | Escherichia Coli. |
| Synonyms | U2 small nuclear ribonucleoprotein A', U2 snRNP A', SNRPA1, Lea1. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPCL TELILTNNSL VELGDLDPLA SLKSLTYLSI LRNPVTNKKH YRLYVIYKVP QVRVLDFQKV KLKERQEAEK MFKGKRGAQL AKDIARRSKT FNPGAGLPTD KKKGGPSPGD VEAIKNAIAN ASTLAEVERL KGLLQSGQIP GRERRSGPTD DGEEEMEEDT VTNGS. |
| Stability/Storage | ALDAAYCFRNVQDNCCLRPLYIDFKRDLGWKWIHEPKGYNANFCAGACPYLWSSDTQHSRVLSLYNTINPEASASPCCVSQDLEPLTILYYIGKTPKIEQLSNMIVKSCKCS |
| Patent Rights | Store at -20°C. For long term storage freezes in working aliquots at -20°C. Repeated freezing and thawing is not recommended. |
More info
Lea1, SNRPA1, U2 snRNP A', U2 small nuclear ribonucleoprotein A'