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Profilin-4 Human Recombinant ( PFN4 Human )
DescriptionPFN4 Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 149 amino acids (1-129 a.a.) and having a molecular mass of 16.4 kDa. PFN4 protein is fused to a 20 amino acid His tag at N-terminus and
Data sheet
| Formulation | PFN4 Human solution containing 20mM Trsi HCL pH-8, 1mM DTT and 10% glycerol. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Description | PFN4 Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 149 amino acids (1-129 a.a.) and having a molecular mass of 16.4 kDa. PFN4 protein is fused to a 20 amino acid His tag at N-terminus and purified by standard chromatography. |
| Protein Background | PFN4 is a small actin-binding protein that participates in the dynamic turnover and restructuring of the actin cytoskeleton. PFN4 is localized in all eukaryotic organisms in the majority of cells. PFN4 is crucial for spatially and temporally controlled growth of actin microfilaments, which is a necessary process in cellular locomotion and cell shape changes. |
| Expression host | Escherichia Coli. |
| Synonyms | PFN-4, Profilin-IV, Profilin4. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Stability | PFN4 Human although stable at 4°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MSHLQSLLLD TLLGTKHVDS AALIKIQERS LCVASPGFNV TPSDVRTLVN GFAKNPLQAR REGLYFKGKDYRCVRADEYS LYAKNENTGV VVVKTHLYLL VATYTEGMYP SICVEATESL GDYLRKKGS. |
| Cleavage Conditions | 50mM Tris-HCl, pH-7.0 (at 25°C), 150mM NaCl, 1mM EDTA, 1mM dithiothreitol. Chill to 5°C prior to use. |
| A280 of 0.1% solution | For Cleavage of a Fusion Protein: During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease. |