More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Rat MMP-2 standard: 2 vials
2. One 96-well plate precoated with anti- Rat MMP-2 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Matrix metalloproteinase-2 (MMP-2), also known as 72 kDa type IV collagenase, Gelatinase A, is a member of the matrix metalloproteinases (MMPs) family belonging to the zinc and calcium dependent endopeptidase superfamily. Matrix Metalloproteinases (MMPs), also called Matrixins, play an important role in the degradation of the extracellular matrix which is involved in a series of physiological processes related to embryonic development, morphogenesis, and tissue remodeling. Human MMP-2 is secreted as a non-glycosylated proenzyme (pro-MMP-2) composed of an 80 amino acid (aa) propeptide and a 551 aa mature polypeptide. The propeptide contains a cysteine switch motif that is conserved in MMPs and keeps MMP-2 in an inactive state. The mature MMP-2 is generated from the pro-MMP-2 by removing the propeptide by means of membrane-type MMPs (MT-MMPs) or the serine proteases Thrombin and activated Protein C. Both the pro- and active forms of human MMP-2 share 96-97% aa sequence identity with mouse and rat pro-MMP-2. MMP-2 is expressed ubiquitiously and participitates in various biological processes such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation. MMP-2 degrades Gelatin and type IV Collagen, cooperated with MMP-9 (Gelatinase B). It can also degrade the matrix proteins Collagens V, VII and X, Decorin, Elastin, and Fibronectin. It has been shown that MMP-2 regulates the processing and activities of many other vasoactive and proinflammatory molecules such as Adrenomedullin, Big Endothelin-1, Calcitonin gene-related peptide, CCL7/MCP-3, CXCL12/SDF-1, Galectin-3, IGFBP-3, IL-1β, S100A8, and S100A9.