More info
Assay Range | 15.6-1,000 pg/mL |
Sensitivity | 1.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Rat IL-13 standard: 2 vials
2. One 96-well plate coated with anti- Rat IL-13 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Interleukin 13 (IL-13) is a cytokine that is encoded by the IL13 gene in humans. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils. Structurally and functionally, IL-13 is closely related to cytokine IL-4. Mature rat IL-13 shares 59% and 75% amino acid sequence identity with human and mouse IL-13, respectively. Rat IL-13 exhibits species cross-reactivity in human and mouse.
IL-13 plays a critical role in the pathogenesis of allergic asthma and atopy and exhibits diverse activities on different cell types. On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNFα production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 Rα1, triggering IL-13 Rα1 association with IL-4 Rα. IL-13 also binds with high affinity to IL-13 Rα2 which is expressed on the cell surface and as a soluble molecule. IL-13 Rα2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies.