LACTB E.coli

Data sheet

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM Phosphate buffer pH-7.

Solubility

It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Description

Recombinant E.coli Beta-Lactamase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids and having a molecular mass of 29 kDa. Beta Lactamase is purified by proprietary chromatographic techniques.

Protein Background

Beta-lactamase is a type of enzyme (EC 3.5.2.6) produced by some bacteria that is responsible for their resistance to beta-lactam antibiotics like penicillins, cephalosporins, cephamycins and carbapenems. These antibiotics have a common element in their m

Expression host

Escherichia Coli.

Synonyms

b-Lactamase, EC 3.5.2.6, TEM precursor.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Beta Lactamase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Beta Lactamase Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.For long term stor

Amino acid sequence

MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVRELCSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSR

Unit Definition

One unit will hydrolyze 1.0 mole of indicated substrate per min at pH 7.0 at 25°C. The International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Specific Activity

700IU/mg.