IL 13 Rhesus Macaque

Data sheet

Formulation

Lyophilized from a 0.2?m filtered concentrated solution in PBS, pH 7.4 containing 5% trehalose.

Solubility

It is recommended to reconstitute the lyophilized IL-13 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by SDS-PAGE and HPLC analyses.

Description

IL 13 Rhesus Macaque Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 114 amino acids and having a molecular mass of 12.6kDa.The IL 13 Rhesus Macaque is purified by proprietary chromatographic techniques.

Protein Background

IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells.

Expression host

Escherichia Coli.

Synonyms

NC30, ALRH, BHR1, P600, IL-13, MGC116786, MGC116788, MGC116789.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized IL-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-13 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Amino acid sequence

SPSPVPRSTA LKELIEELVN ITQNQKAPLC NGSMVWSINL TAGVYCAALE SLINVSGCSA IEKTQRMLNG FCPHKVSAGQ FSSLRVRDTK IEVAQFVKDL LVHLKKLFRE GRFN

Biological Activity

The ED50 as determined by the dose-dependent proliferation of TF-1 cells was 11,000,000 units/mg.