| Storage |
HEV ORF2 protein although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Formulation |
20mM Tris-HCl pH-8, 8M urea and 10mM B-ME. |
| Purity |
HEV ORF2 Protein is >95% pure as determined by 10% PAGE (coomassie staining). |
| Applications |
HEV ORF2 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HEV with minimal specificity problems. |
| Description |
The e.coli derived HEV protein is fused with beta-galactosidase at the N-Terminus and contains the HEV immunodominant regions from ORF2 403-461 a.a. |
| Protein Background |
Hepatitis E virus (HEV), the major etiologic agent of enterically transmitted non-A, non-B hepatitis worldwide, is a spherical, non-enveloped, single stranded RNA virus that is approximately 32 to 34 nm in diameter. HEV belongs to a genus of HEV-like viruses (unassigned genus). HEV has a single-stranded polyadenylated RNA genome of approximately 8 kb. Based on its physicochemical properties it is presumed to be a calici-like virus. |
| Purification Method |
HEV ORF2 was purified by proprietary chromatographic technique. |
| Advantages |
Blood samples from each donor have been tested and found negative for HBsAg, anti-HCV, HIV Ag/Ab, and syphilis. |
