| Formulation |
The CXADR solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 10% glycerol. |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Description |
CXADR Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 241 amino acids (20-237) and having a molecular mass of 26.0kDa.CXADR is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
CXADR is a component of the epithelial apical junction complex which operates as an homophilic cell adhesion molecule and is crucial for tight junction integrity. CXADR also takes part in transepithelial relocation of leukocytes through adhesive interactions with AMICA1/JAML a transmembrane protein of the plasma membrane of leukocytes. A number of transcript variants encoding different isoforms were identified for this gene. Pseudogenes of this gene were located on chromosomes 15, 18, and 21. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Coxsackie Virus And Adenovirus Receptor, CAR, 46 KD Coxsackievirus And Adenovirus Receptor (CAR) Protein 11, Coxsackievirus B-Adenovirus Receptor, HCVADR, CVB3-Binding Protein, CAR4/6, HCAR. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSLSITTPE EMIEKAKGET AYLPCKFTLS PEDQGPLDIE WLISPADNQK VDQVIILYSG DKIYDDYYPD LKGRVHFTSN DLKSGDASIN VTNLQLSDIG TYQCKVKKAP GVANKKIHLV VLVKPSGARC YVDGSEEIGS DFKIKCEPKE GSLPLQYEWQ KLSDSQKMPT SWLAEMTSSV ISVKNASSEY SGTYSCTVRN RVGSDQCLLR LNVVPPSNKA G. |
| Preparation protocol |
Protocol to prepare dengue IgG/IgM rapid test:1. Prepare 532 to 534nm colloid gold, conjugation within 24 hours after colloid gold preparation;2. Adjust colloid gold OD at 1.3 to 1.4, conjugation concentration and pH are 16 to 18 ug/ml gold and 7.2;3. Shanking conjugation for 6-8 hours, then centrifuge the conjugation at 10,000 rpm at 18oC for 20 min;4. Remove the supernatnant as more as possible, add thesupplied gold suspension buffer;5. Add thesupplied blocking reagentto the final concentration to 0.4%;6. Blocking for 1 hours , then add sucrose to the final concentration to 20%;7. Dipping the conjugated gold to the gold pad ( do not use sodium phosphate for gold pad treatment);8. After drying, assemble gold pad, thesupplied sample padand absorbent pad over backing card;9. Add 10ul serum/plasma and add two drops running buffer for assay. |
