| Formulation |
CUZD1 was filtered (0.4um) and lyophilized in phosphate buffered saline and 5% (w/v) trehalose. |
| Solubility |
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. CUZD1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Description |
CUZD1 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Glu25-Ser568) containing a total of 554 amino acids, having a calculated molecular mass of 62.2kDa and fused to a 10 aa His tag at C-Terminus. |
| Protein Background |
CUB and Zona Pellucida-Like Domains 1 (CUZD1) antiserum inhibits cell attachment and proliferation of ovarian cancer cells therefore may be involved in these processes. In addition, CUZD1 may also have a role in the uterus during late pregnancy and/or in trypsin activation in pancreatic acinar cells. |
| Expression host |
HEK 293. |
| Synonyms |
CUB and Zona Pellucida-Like Domains 1, CUB and ZP Domain-Containing Protein 1, Transmembrane Protein UO-44, Estrogen Regulated Gene 1, ERG-1, UO-44, CUZD1. |
| Reagent Appearance |
Filtered White lyophilized (freeze-dried) powder. |
| Stability |
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Amino acid sequence |
EAEGNASCTV SLGGANMAET HKAMILQLNP SENCTWTIER PENKSIRIIF SYVQLDPDGS CESENIKVFD GTSSNGPLLG QVCSKNDYVP VFESSSSTLT FQIVTDSARI QRTVFVFYYF FSPNISIPNC GGYLDTLEGS FTSPNYPKPH PELAYCVWHI QVEKDYKIKL NFKEIFLEID KQCKFDFLAI YDGPSTNSGL IGQVCGRVTP TFESSSNSLT VVLSTDYANS YRGFSASYTS IYAENINTTS LTCSSDRMRV IISKSYLEAF NSNGNNLQLK DPTCRPKLSN VVEFSVPLNG CGTIRKVEDQ SITYTNIITF SASSTSEVIT RQKQLQIIVK CEMGHNSTVE IIYITEDDVI QSQNALGKYN TSMALFESNS FEKTILESPY YVDLNQTLFV QVSLHTSDPN LVVFLDTCRA SPTSDFASPT YDLIKSGCSR DETCKVYPLF GHYGRFQFNA FKFLRSMSSV YLQCKVLICD SSDHQSRCNQ GCVSRSKRDI SSYKWKTDSI IGPIRLKRDR SASGNSGFQH ETHAEETPNQ PFNS HHHHHH HHHH. |
| Preparation protocol |
Protocol to prepare dengue IgG/IgM rapid test: 1. Prepare 532 to 534nm colloid gold, conjugation within 24 hours after colloid gold preparation; 2. Adjust colloid gold OD at 1.3 to 1.4, conjugation concentration and pH are 16 to 18 ug/ml gold and 7.2;3. Shanking conjugation for 6-8 hours, then centrifuge the conjugation at 10,000 rpm at 18oC for 20 min;4. Remove the supernatnant as more as possible, add the supplied gold suspension buffer;5. Add the supplied blocking reagent to the final concentration to 0.4%;6. Blocking for 1 hours , then add sucrose to the final concentration to 20%;7. Dipping the conjugated gold to the gold pad ( do not use sodium phosphate for gold pad treatment);8. After drying, assemble gold pad, the supplied sample pad and absorbent pad over backing card; 9. Add 10ul serum/plasma and add two drops running buffer for assay. |
