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HIV-1 gp120 ( PNDmn ), Mouse antibody ( HIV-1 gp120 antibody )
TypeMouse antibody Monoclonal.CloneNYRHIV1gp120.Immunogenr.gp120 (MN strain).Ig Subclassmouse IgG1.Purification MethodIon exchange column.Protein concentration1mg/ml in PBS (after reconstitution).Shipping ConditionsAntibody is shipped lyophilized at
Data sheet
| Clone | NYRHIV1gp120. |
| Formulation | Polyclonal Antibody serum was lyophilized in vials. |
| Solubility | The lyophilized antibody should be reconstituted in sterile dH2O. The amount of sterilized dH2O can vary depending on the final concentration desired. |
| Purity | >95% as estimated by SDS-PAGE. |
| Applications | Binds to recombinant HIV-1 gp120 ELISA and Western Blot Optimal dilutions should be determined by the customer, based on titration. Suggested starting dilutions are listed below: Western Blot: 1-500 to 1-1000. ELISA: 1:500 to 1000. |
| Description | Rabbit Polyclonal Antibody to gp120 (HIV-1) serum. |
| Protein Background | Human immunodeficiency virus (HIV) is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells(specifically CD4+ T cells), macrophagesand dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosisin infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytesthat recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunityis lost, and the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genomeis converted to double-stranded DNAby a virally encoded reverse transcriptasethat is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integraseso that the genome can be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latentand the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated that can then infect other cells. |
| Synonyms | HIV-1 gp120, HIV1gp120, HIV-I gp120, HIVIgp120. |
| Reagent Appearance | Sterile filtered pinkish powder. |
| Stability | Stable at -20°C. Avoid freeze/thaw cycles. |
| Type | Mouse antibody Monoclonal. |
| Immunogen | r.gp120 (MN strain). |
| Ig Subclass | mouse IgG1. |
| Purification Method | Ion exchange column. |
| Storage Procedures | In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC. |