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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| stratifin | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma(140-156aa KKRIIDSARSAYQEAMD), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SFN |
Protein | 14-3-3 protein sigma |
Uniprot ID | P31947 |
Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. |
Tissue Specificity | Present mainly in tissues enriched in stratified squamous keratinizing epithelium. |
Sub-cellular localization | Cytoplasm. Nucleus . Secreted. Note: May be secreted by a non-classical secretory pathway. |
Sequence Similarities | |
Aliases | 14 3 3 protein sigma antibody|14-3-3 protein sigma antibody|1433S_HUMAN antibody|Epithelial cell marker protein 1 antibody|Er antibody|HME 1 antibody|HME1 antibody|MGC143283 antibody|Mkrn3 antibody|Mme1 antibody|OTTHUMP00000004242 antibody|RP23 137L22.11 antibody|SFN antibody|SFN protein antibody|Stratifin antibody|YWHAS antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutions ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutions IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutions IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-14-3-3 sigma antibody, ASA-B0002, Western blotting
All lanes: Anti 14-3-3 sigma (ASA-B0002) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: SE620 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
All lanes: Anti 14-3-3 sigma (ASA-B0002) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: SE620 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
Anti-14-3-3 sigma antibody, ASA-B0002, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti-14-3-3 sigma antibody, ASA-B0002, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue