More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mesothelin | Polyclonal | IgG | Rabbit | Human | ELISA | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Mesothelin recombinant protein (Position: K306-L576). Human Mesothelin shares 57% and 56% amino acid (aa) sequence identity with mouse and rat Mesothelin, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MSLN |
Protein | Mesothelin |
Uniprot ID | Q13421 |
Function | Membrane-anchored forms may play a role in cellular adhesion. |
Tissue Specificity | Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). |
Sub-cellular localization | Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus. |
Sequence Similarities | |
Aliases | CAK 1 antibody|CAK1 antibody|CAK1 antigen antibody|cleaved form antibody|Megakaryocyte potentiating factor antibody|Mesothelin antibody|Mesothelin isoform 1 precursor antibody|MPF antibody|Msln antibody|MSLN_HUMAN antibody|Pre pro megakaryocyte potentiating factor antibody|Pre-pro-megakaryocyte-potentiating factor antibody|SMR antibody|SMRP antibody|Soluble MPF mesothelin related protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Mesothelin antibody, ASA-B1266, Western blotting
All lanes: Anti Mesothelin (ASA-B1266) at 0.5ug/ml
WB: Recombinant Human Mesothelin Protein 0.5ng
Predicted bind size: 43KD
Observed bind size: 43KD
All lanes: Anti Mesothelin (ASA-B1266) at 0.5ug/ml
WB: Recombinant Human Mesothelin Protein 0.5ng
Predicted bind size: 43KD
Observed bind size: 43KD
Anti- Mesothelin antibody, ASA-B1266,IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
