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Data sheet
| Background | Cytomegalovirus is a herpes virus and a leading biological factor causing congenital abnormalities and complications among those who receive massive blood transfusions and immunosuppressive therapy. Serological tests for detecting the presence of antibody to CMV can provide valuable information regarding the history of previous infection, diagnosis of active or recent infection, as well as in screening blood for transfusions in newborns and immuno-compromised recipients. CMV IgG ELISA is an accurate serologic method to detect CMV antibody for identification of CMV infection. |
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Size |
96T |
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Specificity |
No cross-reaction with other related substances detected |
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Storage |
Store at 2 - 8ºC. |
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Assay Principle |
Direct ELISA/Semi-Quantitative Detection |
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Sample Volume |
100 µL final volume |
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Sample Type |
Serum, 5 µL |
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Detection Method |
Chromogenic |
Kit Components
1. One 96-well plate pre-coated with CMV antigen
2. Negative Control: 1 vial
3. Positive Control: 1 vial
4. Calibrator: 4 vials
5. Diluent buffer: 22 ml
6. Anti- porcine IgG -HRP Conjugate: 1 vial
7. TMB developing agent: 11 mL x1
8. Stop solution: 11 mL x1
9. Washing solution (20x): 1 vial
Assay principle
This assay employs the enzyme-linked immunosorbent assay (ELISA) technology to detect CMV IgG in the samples. Purified CMV antigen was precoated in microplate wells. The CMV IgG in the sample, if presents, will bind to the antigen immobilized on the wells and interacts with anti- porcine IgG conjugated with HRP to form an immunocomplex. Following incubation and washing procedures to remove unbound substances, this reaction is visualized by the addition of the chromogen tetramethylbenzidine (TMB). After stopping the reaction with sulfuric acid, the blue color turns yellow. What can be measured at this point is the amount of color intensity proportional to the amount of antibody captured in the wells, and to the sample.