Data sheet
| Assay Range | 12.5 - 800 pg/ml |
More info
Sensitivity
10 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse CD62L standard: 2 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse CD62L Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse CD62L Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
L-selectin, also known as CD62L, is a cell surface component that is a member of a family of adhesion/homing receptors that play important roles in lymphocyte-endothelial cell interactions. It is expressed in B-cell lines and T-lymphocytes and belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups.CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4-binding proteins. CD62L mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes and promotes initial tethering and rolling of leukocytes in endothelia.
