| Specificity |
reactive with Mouse Neprilysin, reactivity with other related proteins not observed |
| Assay Range |
93.75-6000 pg/ml |
| Sensitivity |
9 pg/ml |
| Size |
96 Tests |
| Storage |
2-8℃ |
| Background |
Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), CD10, and common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably the amyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. Synthesized as a membrane-bound protein, the neprilysin ectodomain is released into the extracellular domain after it has been transported from the Golgi apparatus to the cell surface. In neurons, neprilysin is regulated by the protein nicastrin, a component of the gamma secretase complex that performs a necessary step in processing amyloid precursor protein to amyloid beta. |
| Assay principle |
This Mouse Neprilysin ELISA Kit was based on standard sandwich enzyme-linked immunosorbent assay technology. Mouse Neprilysin specific antibodies were precoated onto 96-well plates. The Mouse Neprilysin specific detection antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP was added and unbound conjugates were washed away with wash buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow color is proportional to the Mouse Neprilysin amount of sample captured in plate. |
| Sample type |
serum, plasma, cell culture supernatant |
| Assay duration |
4 hours |
| Assay format |
Sandwich |
| Alternative Name of Analyte |
MME；CALLA；CD10；NEP；SFE |
