| Specificity |
reactive with Mouse MFG-E8, reactivity with other related proteins not observed |
| Assay Range |
31.25-2000 pg/ml |
| Sensitivity |
3 pg/ml |
| Size |
96 Tests |
| Storage |
2-8℃ |
| Background |
Milk fat globule-EGF factor 8 protein Mfge8), also known as lactadherin, is a protein which is encoded by the MFGE8 gene. |
| Assay principle |
This Mouse MFG-E8 ELISA Kit was based on standard sandwich enzyme-linked immunosorbent assay technology. Mouse MFG-E8 specific antibodies were precoated onto 96-well plates. The Mouse MFG-E8 specific detection antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP was added and unbound conjugates were washed away with wash buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow color is proportional to the Mouse MFG-E8 amount of sample captured in plate. |
| Sample type |
serum, plasma, cell culture supernatant |
| Assay duration |
4 hours |
| Assay format |
Sandwich |
| Alternative Name of Analyte |
MFGE8；BA46；EDIL1；HMFG；HsT19888；MFG-E8；MFGM；OAcGD3S；SED1；SPAG10；hP47 |
