Data sheet
| Assay Range | 7.8 - 500 pg/ml |
| Background | Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6's role as an anti-inflammatory myokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. IL-6 is also considered a myokine, a cytokine produced from muscle, which is elevated in response to muscle contraction. It is significantly elevated with exercise, and precedes the appearance of other cytokines in the circulation. During exercise, it is thought to act in a hormone-like manner to mobilize extracellular substrates and/or augment substrate delivery. IL-6 had previously been classified as a proinflammatory cytokine. Therefore, it was first thought that the exercise-induced IL-6 response was related to muscle damage.However, it has become evident that eccentric exercise is not associated with a larger increase in plasma IL-6 than exercise involving concentric "nondamaging" muscle contractions. This finding clearly demonstrates that muscle damage is not required to provoke an increase in plasma IL-6 during exercise. As a matter of fact, eccentric exercise may result in a delayed peak and a much slower decrease of plasma IL-6 during recovery. |
| Alternate Names | IL6, BSF2, HGF, HSF, IFNB2, IL-6, BSF-2, CDF, IFN-beta-2, interleukin 6 |
| Dilution Factors | Undiluted or user test to decide. |
| Gene ID | 16193 |
| UniProt ID | P08505 |
More info
Assay Format
Sandwich ELISA.
Storage
Store at 4 °C.
Expiration
12 months at 4 °C.
Range
7.8 pg/mL - 500 pg/mL.
Sensitivity
5 pg/mL.
Specificity
No detectable cross-reactivity with any other cytokine.
Application
cell culture supernatants, serum and plasma.
Kit Components:
1. Lyophilized recombinant Mouse IL-6 standard: 0.5 ng/tube—2.
2. One 96-well plate precoated with anti-Mouse IL-6 antibody.
3. Sample diluent buffer: 12 mL —2
4. Biotinylated anti-Mouse IL-6 antibody: 60µL, dilution 1:180.
5. Antibody diluent buffer: 12ml.
6. Streptavidin-HRP: 300 µL, dilution 1:40.
7. Streptavidin-HRP diluent buffer: 12ml.
8. Chromogen A: 6ml.
9. Chromogen B: 6ml
10. Stop Solution: 6ml.
Related Products:
For more sensitive mouse IL-6 detection, or try to save mouse serum/plasma samples by using small volume, try high-sensitivity mouse IL-6 ELISA kit Cat#NR-HS10596:
http://www.novateinbio.com/home/1166283-mouse-il-6-elisa-kit-high-sensitivity-.html