Data sheet
| Background | The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). IL-17A, often referred to as IL-17, was originally discovered at transcriptional level by Rouvier et al. in 1993 from a rodent T-cell hybridoma, derived from the fusion of a mouse cytotoxic T cell clone and a rat T cell lymphoma. Human and mouse IL-17A were cloned a few years later by Yao and Kennedy. Lymphocytes including CD4+, CD8+, gamma-delta T (γδ-T), invariant NKT and innate lymphoid cells (ILCs) are primary sources of IL-17A. Non-T cells, such as neutrophils, have also been reported to produce IL-17A under certain circumstances. IL-17A producing T helper cells (Th17 cells) are a distinct lineage from the Th1 and Th2 CD4+ lineages and the differentiation of Th17 cells requires STAT3[11] and RORC. IL-17A receptor A (IL-17RA) was first isolated and cloned from mouse EL4 thymoma cells and the bioactivity of IL-17A was confirmed by stimulating the transcriptional factor NF-kappa B activity and interleukin-6 (IL-6) secretion in fibroblasts. IL-17RA pairs with IL-17RC to allow binding and signaling of IL-17A and IL-17F. High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The presence of IL-17 has been proven in a number of ocular diseases associated with neovascularization. Elevated concentration of IL-17 have been shown in vitreous fluid during proliferative diabetic retinopathy. Increased rates of Th17 cells and higher concentrations of IL-17 have been observed in patients with age-related macular degeneration. |
| Alternate Names | IL17A, CTLA8, IL-17, IL-17A, IL17, CTLA-8, interleukin 17A |
| Gene ID | 16171 |
| UniProt ID | Q62386 |
More info
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Assay Range |
15.6--1000 pg/mL |
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Sensitivity |
1.0 pg/mL |
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Specificity |
No cross-reaction with other related substances detected |
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Size |
96T |
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Storage |
Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
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Assay Principle |
Sandwich ELISA |
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Sample Volume |
100 µL final volume, dilution factor varies on samples |
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Sample Type |
serum, plasma or cell culture supernatant |
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Detection Method |
Chromogenic |
Kit Components
1. Recombinant Mouse IL-17 standard: 1 ng x 2 vials
2. One 96-well plate coated with Mouse IL-17 Ab
3. Sample diluent buffer: 12 mL - 2
4. Detection antibody: 60 µL, 1:180
5. Streptavidin-HRP: 300 µL, 1:40
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 6 ml TMB x1, 6 ml H2O2 x1.
9. Stop solution: 6 mL x1
10. Washing solution (20x): 25 mL x1
For more sensitive IL-17A detection or try to save mouse plasma/serum sample volume, use more sensitive kit: Cat#NP-HS10041, http://www.novateinbio.com/home/1166285-mouse-il-17a-elisa-kit-high-sensitivity.html