More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse CRP standard: 2 vials.
2. One 96-well plate precoated with anti- Mouse CRP Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
C-Reactive Protein (CRP), also known as Pentraxin 1, is a member of the Pentraxin family that also includes Pentraxin 2/SAP and Pentraxin 3/TSG-14. CRP assembles non-covalently into a 110-120 kDa cyclical pentamer. Mature mouse CRP shares 71% amino acid sequence identity with human and rat CRP.
In humans, CRP is a major acute-phase protein. Its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds and opsonizes apoptotic cells as well as bacteria such as S. pneumoniae. It subsequently enhances the phagocytosis of these opsonized cells. In addition, CRP binds several proteins in the complement cascade including C1q, C4BP, and Factor H. It enhances activation of the classical complement pathway and the deposition of C3b. In later stages of the response, CRP inhibits complement- mediated cell lysis through its binding to C4BP and Factor H. These interactions induce the upregulation of complement inhibitory proteins CD46, CD59, and CD55/DAF, and inhibit assembly of the membrane attack complex (MAC). Fc receptors are required for the phagocytosis of CRP-opsonized target cells. CRP binding to FcγRI induces Src activation which subsequently triggers the inhibitory FcγRIIB and dampens the inflammatory response. CRP additionally promotes dendritic cell maturation and humoral immunity.