More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse CHI3L1 standard: 2 vials
2. One 96-well plate precoated with anti- Mouse CHI3L1 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Chitinase 3-like 1 (CHI3L1), also known as 39 kDa synovial protein, cartilage glycoprotein 39 (HCGP39), chondrex, and YKL-40, is an approximately 40kDa secreted glycoprotein encoded by the CHI3L1 gene in humans. The name YKL-40 is derived from the three N-terminal amino acids present on the secreted form and its molecular mass. It belongs to the glycosyl hydrolase family 18 and is analogous to chitinases, while it lacks chitinase activity. Chitinase is a group of enzymes capable of cleaving chitin, an abundant polysaccharide present in many organisms such as the exoskeleton of insects and walls of fungi. Mouse CHI3L1 shares 77% and 74% identity with rat and human counterpart, respectively.
CHI3L1 is expressed by many different cell types, such as monocytes, macrophages, endothelial cells, and chondrocytes. It has been shown that CHI3L1 is able to suppress matrix metalloproteinase production, such as MMP-1, MMP-3, and MMP-13, induced by IL-1 and TNF-α. CHI3L1 can also promote the growth of skin fibroblasts and synovial cells by itself or synergistically with IGF-I through activating MAPK and protein kinase B signaling cascades. In addition, it can facilitate vascular endothelial cell attachment, migration, and branching. Clinically, Microarray gene analysis showed that CHI3L1 is one of the most over-expressed genes in glioblastoma, papillary thyroid carcinoma, and extracellular myxoid chondrosarcoma. It is also upregulated in a number of solid tumors, such as cancer of the breast, colon, lung, kidney, and ovary. In cancer patients, serum CHI3L1 concentrations are often predictive of tumor stages, response to therapy, and prognosis. Elevated serum CHI3L1 levels have also been observed in some conditions of inflammation and tissue remodeling, such as arthritis, severe bacterial infection, inflammatory bowel disease, and liver cirrhosis.