More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse TNFRSF7 standard: 2 vials
2. One 96-well plate precoated with anti-Mouse TNFRSF7 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
CD27, also known as CD27L receptor, T-cell activation antigen CD27, T14, tumor necrosis factor receptor superfamily member 7 (TNFRSF7), is a transmembrane glycoprotein belonging to the TNF receptor superfamily. Mature mouse CD27 consists of a 159 amino acid (aa) extracellular domain (ECD) with three TNFR cysteine-rich repeats, a 21 aa transmembrane segment, and a 47 aa cytoplasmic domain. The extracellular domain of mouse CD27 is 63% and 85% identical to the human and rat CD27, respectively. CD27 is weakly expressed on naïve T cells and NK cells. It is induced on activated germinal center B cells, plasma cells, and a subset of memory B cells. On activated B cells, activated T cells, and dendritic cells , CD27 binds to the transmembrane glycoprotein CD27 Ligand/CD70 to initiate the activation and survival of CD4+ helper T cell , CD8+ effector T cells, memory T cells , as well as NK cells, and promote germinal center formation and the expansion and affinity maturation of memory B cell responses. Therefore, CD27 plays a key role in regulating B-cell activation and immunoglobulin synthesis.