More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse MMP-3 standard: 2 vials
2. One 96-well plate precoated with anti- Mouse MMP-3 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Matrix metalloproteinase-3 (MMP-3), also known as Stromelysin-1, is a member of the matrix metalloproteinases (MMPs) family belonging to the zinc and calcium dependent endopeptidase superfamily. Matrix Metalloproteinases (MMPs), also called Matrixins, plays an important role in the degradation of the extracellular matrix which is involved in a series of physiological processes related to embryonic development, morphogenesis, and tissue remodeling. Human MMP-3 is secreted as a non-glycosylated proenzyme (pro-MMP-3) composed of a propeptide with a cysteine switch motif that is conserved in MMPs and keeps MMP-3 in an inactive state, and a mature polypeptide. The mature MMP-3 is generated from the pro-MMP-3 by removing the propeptide by means of different proteases such as itself, chyrotrypsin, neutrophil elastase and plasma kallikrein. Expression of mouse MMP-3 is induced in fibroblasts, chondrocytes, endothelial cells, macrophages, vascular smooth muscle cells, osteoblasts, and keratinocytes in response to stimuli of IL-1, TNF-α, EGF, PDGF, phorbol and oncogenic cellular transformation. In contrast, retinoic acid, glucocorticoids, estrogen, progesterone and TGF-β inhibit the production of MMP-3. The active MMP-3 can degrade types III, IV, IX and X collagen, aggrecan, fibronectin, laminin, IGFBP-3, serpins, and IL-1β.