More info
Assay Range | 15.6--1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Sample Type | serum, plasma or cell culture supernatant |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse IL-12(p70) standard: 2 vials
2. One 96-well plate coated with Mouse IL-12(p70) Ab
3. Sample diluent buffer: 12 ml— 2
4. Detection antibody: 130 µL , dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Interleukin 12 (IL-12), also known as NKSF or p70, is a 70-75 kDa heterodimeric glycoprotein belonging to the IL-12 family. IL-12 is encoded by two separate genes, IL-12A and IL-12B, in human. IL-12 is composed of two disulfide-linked subunits, p35 and p40. The mature p35 subunit is synthesized as a 197 amino acid (aa) containing seven cysteines and one potential N-linked glycosylation site. Mature human p35 shares 58% aa identity with mouse and rat p35. Mature human p40 is a 306 aa protein with 11 cysteines and three potential N-linked glycosylation sites. Mature human p40 is 66% aa identical to mouse and rat p40. IL-12 is produced in macrophages and dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, plasmacytoid dendritic cells, and microglia.
Human IL-12 receptor is composed of at least two type I transmembrane glycoproteins, Rβ1 and Rβ2. Rβ1 is the principal binding protein for the p40 , while Rβ2 might be the principal signal transduction component and serve as an attachment point for a disulfide-linked p35-p40 heterodimer. IL-12 plays an important role in regulating the activities of natural killer cells and T lymphocytes. It enhances cytotoxic activity and induces IFN-γ production in NK cells, T cells and dendritic epidermal T cells.