More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 5.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse Cathepsin B standard: 2 vials.
2. One 96-well plate precoated with anti- Mouse Cathepsin B Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
The cathepsins are a group of lysosomal proteases that are active in acidic environments and play an important role in protein degradation. Currently, 11 human cathepsins (B, C, F, H, K, L, O, S, V, W and X) have been shown to have broad substrate specificity. For example, Cathepsins A and G are serine proteases and cathepsins D and E are aspartic proteases. Cathepsins are synthesized as inactive proenzymes and processed to become mature and active enzymes. Human Cathepsin B precursor consists of 339 amino acid (aa) residues containing a signal peptide, a pro- peptide and a mature chain. Cathepsin B is capable of digesting a number of important molecules, such as pro- and active caspases, pro-renin, and secretory leukocyte protease inhibitor (SLPI), therefore, it may play a key role in in apoptosis, angiotensin production, and progression of emphysema. It is reported that deficiency of Cathepsin B and L in mice is lethal at two to four weeks of age and characterized by neuronal loss and brain atrophy, suggesting that Cathepsin B and L are critical to maintenance of the central nervous system. In addition, expression of Cathepsin B in human and animal tumors is upregulated and redistributed, making it a potentially useful marker for invasion and metastasis of various cancers, such as advanced melanoma, breast cancer, and colorectal cancer.