View larger
Mouse Neuron Specific Enolase NSE ELISA Kit
BG-MUS11622
Mouse Neuron Specific Enolase ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction
Data sheet
| Storage | 2 - 8 ℃ |
| Background | Enolases are glycolytic enzymes that catalyze the conversion of 2-phosphoglycerate into phosphoenolpyruvate. This catalysis is dependent on two Mg2+ ions bound to the catalytic active site of the enolase. Vertebrates express three different enolase isoforms that consist of three different monomeric units forming either homo- or heterodimers. As NSE is normally expressed in a tissue-specific manner, an increased NSE concentration in serum or cerebrospinal fluid concentration often indicates neuronal damage or malignancies. In clinical diagnostics, NSE is thus a widely used marker for neuroendocrine tumors (NETs), including neuroblastoma and small-cell lung cancer (SCLC). NSE level in bodily fluids has been reported to correlate with extent of disease and response to treatment, allowing the marker to be used as a tool for diagnosis, prognosis, as well as treatment follow-up. Identification of NSE in cord blood allows early postnatal confirmation of neuroblastoma diagnosis in newborn babies. Besides cancer, increased NSE may also reflect the presence other conditions, such as brain damage, cerebral accidents, myocardial infarctions, Guillain-Barré syndrome, or CreutzfeldtJakob disease. Hemolysis can occasionally cause false positive test results, because erythrocytes also contain NSE. |
| Alternate Names | ENO2; enolase 2 (gamma, neuronal); Enolase 2; gamma-Enolase; Neural enolase; neuron specific gamma enolase; neurone-specific enolase; Neuronspecific Enolase; Neuron-specific enolase; NSE |
| Assay Type | Sandwich ELISA |
| Kit Type | Colorimetric |
| Assay Time | 4.5 hours |
| Sample Type | serum, plasma |
| Assay Range | 0.312 - 20 n/ml |
| Sensitivity | 0.069 n/ml |
| Specificity | no cross-reactivity with mouse enolase 1, enolase 3, PGK1 and PKM2. |
| Recovery | 89% to 111% |
| Precision | Inter-assay: 8.4%, Intra-assay: 4.7% |
| Dilution Factors | undiluted |
| Gene ID | 13807 |
More info
Mouse Neuron Specific Enolase ELISA Kit
For protocols and availability questions, please fill out our contact us form and make sure to include your company name, business email, and phone number. We will contact you within 24 hours.
Different lot may vary, please follow the instruction coming with the kit.