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Mouse Vitamin D Binding Protein DBP ELISA Kit
BG-MUS10773
Mouse Vitamin D Binding Protein (DBP) ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction
Data sheet
| Background | Vitamin D-binding protein (DBP), also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene.Human GC is a glycosylated alpha-globulin, ~58 kDa in size. Its 458 amino acids are coded for by 1690 nucleotides on chromosome 4 (4q11–q13). The primary structure contains 28 cysteine residues forming multiple disulfide bonds. GC contains 3 domains. Domain 1 is composed of 10 alpha helices, domain 2 of 9, and domain 3 of 4. Vitamin D-binding protein belongs to the albumin gene family, together with human serum albumin and alpha-fetoprotein. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It is able to bind the various forms of vitamin D including ergocalciferol (vitamin D2) and cholecalciferol (vitamin D3), the 25-hydroxylated forms (calcifediol), and the active hormonal product, 1,25-dihydroxyvitamin D (calcitriol). The major proportion of vitamin D in blood is bound to this protein. It transports vitamin D metabolites between skin, liver and kidney, and then on to the various target tissues. As Gc protein-derived macrophage activating factor it is a Macrophage Activating Factor (MAF) that has been tested for use as a cancer treatment that would activate macrophages against cancer cells. |
| Alternate Names | GC, DBP, DBP/GRD3, HEL-S-51, VDBG, VDBP, Gc-MAF, GcMAF, vitamin D binding protein, DBP-maf, VDB, GC vitamin D binding protein |
| Assay Type | Sandwich ELISA |
| Kit Type | Colorimetric |
| Assay Time | 4.5 hours |
| Sample Type | serum, cell supernatant |
| Assay Range | 0.312 to 20 ng/mL |
| Sensitivity | 0.11 ng/mL |
| Specificity | recognize both native and recombinant DBP |
| Gene ID | 14473 |
| UniProt ID | P21614 |
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Mouse Vitamin D Binding Protein (Dbp) ELISA Kit
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Different lot may vary, please follow the instruction coming with the kit.