More info
Assay Range | 250-20,000 pg/mL |
Sensitivity | 100 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Sample Type | Cell lysates |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse β-catenin standard: 2 vials
2. One 96-well plate coated with Mouse β-catenin Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 1 vial
5. Streptavidin-HRP: 1 vial
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. Chromogenic solution A: 6 mlx1
9. Chromogenic solution B: 6 mlx1
10. Stop solution: 6 mL x1
11. Washing solution (20x): 25 mL x1
Background
β-catenin, also known as Catenin (cadherin-associated protein), beta 1 or CTNNB1, is encoded by the CTNNB1 gene in humans. β-catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. β-catenin is regulated and destroyed by the beta-catenin destruction complex. It has been showed that β-catenin plays an important role in regulating the coordination of cell-cell adhesion and gene transcription. Mutations and overexpression of β-catenin have been associated with many cancers, including hepatocellular carcinoma, colorectal carcinoma, lung cancer, malignant breast tumors, ovarian and endometrial cancer.