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Mouse Bone Morphogenetic Protein 2 BMP2 ELISA Kit

BG-MUS10361

The kit is used to quantify the Mouse BMP-2 in serum, plasma, body fluids, tissue lysate or cell culture supernatant

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$675.00

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Standard range 62.5 - 4000 pg/mL
Sensitivity 2.0 pg/mL
Assay time 4 hours
Validity Six months
Store at 2-8 °C

Assay principle


This Mouse BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immunosorbent
assay technology. Mouse BMP-2 specific antibodies were precoated onto 96-well plates. The
Mouse BMP-2 specific detection antibodies were biotinylated. The test samples and biotinylated
detection antibodies were added to the wells subsequently and then followed by washing the plate.
Streptavidin-HRP was added and unbound conjugates were washed away with wash buffer. HRP
substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to
produce a blue color product that changed into yellow after adding acidic stop solution. The
density of yellow is proportional to the Mouse BMP-2 amount of sample captured in plate.

 

Materials supplied
 
1. Mouse BMP-2 standard: 10 ng/vial -2.
2. One 96-well plate pre-coated with anti- Mouse BMP-2 Ab: 1.
3. Sample diluent buffer: 12 mL - 1.
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100.
6. Antibody diluent buffer: 12 ml.
7. Streptavidin-HRP diluent buffer: 12 ml.
8. TMB developing agent: 10 ml.
9. Stop solution: 10 ml.
10. 20 - Wash Solution: 25 ml.
11. Plate sealer 1.
12. Package insert 1.
 

Background:


Bone morphogenetic protein 2 (BMP-2), also known as bone morphogenetic protein 2A (BMP
2A), is a member of the BMP family of the TGFβ superfamily. Human BMP2 is synthesized as a
396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment,
and a 114 aa mature region. Mature monomeric BMP2 is an 18 kDa glycoprotein with seven
conserved cysteines that form a cysteine knot structure. Proteolytic removal of the propeptide
enables mature BMP2 to form active disulfide linked homodimers and BMP2/ BMP7
heterodimers which are significantly more potent than BMP2 homodimers at inducing bone
formation in vivo. Mature human BMP2 shares 100% aa sequence identity with mouse and rat
BMP2. BMP2 binds to Activin RI (BMPRIA, or BMPRIB) and BMP RII (Activin RIIB) to activate
the downstream signaling pathways. BMP2 play an important role in inducing chondrocyte
proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage
repair.

 

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Mouse BMP2 ELISA Datasheet

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