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Mouse Bone Morphogenetic Protein 6 BMP6 ELISA Kit
BG-MUS10356
Mouse Bone Morphogenetic Protein 6 (Bmp6) ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general
Data sheet
| Storage | 2 - 8 ℃ |
| Background | Bone Morphogenetic Protein 6, also known as Vgr-1, is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor beta (TGF-beta ) superfamily. Mouse BMP‑6 is synthesized as a 510 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C‑terminal mature protein. Biologically active BMP‑6 consists of a disulfide-linked homodimer of the mature proteins. Mature mouse BMP‑6 shares 96% and 98% aa sequence identity with human and rat BMP‑6, respectively. Cellular responses to BMP‑6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors. Glycosylation of BMP‑6 is required for its interaction with Activin RIA (6). BMP‑6 induces the expression of Noggin and is subsequently antagonized by Noggin. BMP‑6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells, chondrocyte maturation, Ang II-induced aldosterone production in the adrenal cortex, hormone production and responsiveness in ovarian granulosa cells, iNOS and TNF-alpha production in macrophages, the cell death of B cells (8), and neurite outgrowth. BMP‑6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant. BMP‑6 is upregulated during prostate cancer progression and promotes tumor cell metastasis to bone. Through interactions with the BMP coreceptor RGM‑C/Hemojuvelin, BMP‑6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload |
| Alternate Names | BMP6; BMP-6; bone morphogenetic protein 6. VGR; VGR1; Vgr-1 |
| Assay Type | Sandwich ELISA |
| Kit Type | Colorimetric |
| Assay Time | 4.5 hours |
| Sample Type | serum, plasma, culture supernatant |
| Assay Range | 156 - 10,000 pg/ml |
| Sensitivity | 51.5 pg/ml |
| Recovery | 87% to 111% |
| Precision | Inter-assay: 9.8%; Intra-assay: 7.9 % |
| Gene ID | 12161 |
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Mouse Bone Morphogenetic Protein 6 (Bmp6) ELISA Kit
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Different lot may vary, please follow the instruction coming with the kit.