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Size | 96T |
Specificity | No cross-reaction with other related substances detected |
Storage | Store at 2 - 8ºC. Keep detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA/Qualitative Detection |
Sample Volume | 100 µL final volume |
Sample Type | Serum or plasma |
Detection Method | Chromogenic |
Kit Components
1. One 96-well plate pre-coated mouse mAchR
2. Negative Control: 1 vial
3. Positive Control: 1 vial
4. Diluent buffer: 30 ml
5. Anti- Mouse IgG -HRP Conjugate: 1 vial
6. TMB developing agent: 12 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (10x): 1 vial
Assay principle
This kit uses a sandwich enzymeâ€linked immunosorbent assay (ELISA) to detect the existence or not of mouse mAchR in samples. Test samples are added into wells preâ€coated with recombinant mouse muscarinic AchR proteins and incubated appropriately. Following washing to remove unbound antibody and other serum components in the wells, add the HRPâ€conjugated anti-mouse IgG to bind the analyte, followed by incubation and washing procedures. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of mouse mAchR in the samples is determined by comparing the OD of the samples to the CUTOFF value.