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Mouse anti-U1RNP/snRNP70 IgG ELISA Kit
BG-MUS10086
Mouse Anti-Ribonucleoprotein Antibody (Anti-Rnp) ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general
Data sheet
| Assay time | 90 min |
| Background | Small nuclear ribonucleoprotein complexes (abbreviated as U-snRNP) are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins (B/B', D1, D2, D3, E, F, G) form a core subparticle that is common to all U-snRNP complexes. Both the U1-specific proteins and the Sm core particle are targets of autoantibodies which classically have been called the RNP and RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity and specificity and is the only way to determine RNP antibodies without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay. Autoantibodies to U1-snRNP are present in 95% of human patients with Mixed Connective Tissue Disease (MCTD) and 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. |
| Assay principle | The wells of mouse U1RNP/SnRNA70 IgG ELISA test are coated with U1RNP/SnRNA70 recombinant antigen. During the first incubation period U1RNP/SnRNA70 IgG if present, will react with U1RNP/SnRNA70 antigen bound to the wells. After addition of anti-mouse IgG HRP conjugate it binds to mouse IgG to form HRP-mouse IgG and U1RNP/SnRNA70 antigen sandwich. All unbound antibodies and unbound HRP conjugate is washed off. Upon addition TMB substrate, the bound enzyme generates color. The intensity of the color is directly proportional to the concentration of anti-U1RNP/SnRNA70 IgG antibodies in the samples. |
| Sample Type | serum, plasma |
| Assay Range | 6.25 - 200 RU/ml |
| Sample Volume | 50 ul |
| Dilution Factors | 1:100 |
More info
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Different lot may vary, please follow the instruction coming with the kit.