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Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse TLR2 standard: 2 vials.
2. One 96-well plate precoated with anti- Mouse TLR2 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Toll-Like Receptor 2 (TLR2), also known as Toll/interleukin-1 receptor-like protein 4 or CD282, is a transmembrane protein belonging to the TLR family. Mouse TLR2 is a 760 amino acid (aa) glycoprotein composed of a 563 aa extracellular region with 16 leucine-rich repeats, a 21 aa TM segment, and a 176 aa cytoplasmic domain. ECD of Mouse TLR2 shares 67% aa sequence identity with mouse and rat TLR2. TLR2 is expressed on antigen presenting cells (plasmacytoid dendritic cells, B cells, monocytes, and macrophages), T cells (activated CD4+, CD8+, memory, and Treg), renal tubule epithelial cells, trophoblasts, and decidual and amnion epithelial cells. The soluble forms of TLR2 function as decoy receptors that suppress TLR2 mediated inflammation but are unable to bind ligand.
TLR2 elicits its effects by interacting with multiple proteins in the plasma membrane including TLR1, TLR6, TLR10, CD14, CD36, CXCR4, Dectin-1, and Integrins αVβ3 and α3β1, as well as the ganglioside GD1a. TLR2-containing receptor complexes are differentially responsive to microbial lipopeptides, peptidoglycans, lipoteichoic acid, enterotoxin, LPS, as well as yeast and parasite glucans. TLR2 is also activated by endogenous ligands such as amyloid Aβ peptide oligomers, Serum Amyloid A, HMGB1, beta-Defensin 3, PAUF, Biglycan, and LMW Hyaluronan. Ligand binding by TLR2 can induce receptor association with CXCR4, resulting in inhibition of CXCR4 and TLR2 signaling. TLR2 initiates proinflammatory signaling through its cytoplasmic TIR domain. TLR2 signaling also regulates adaptive immune responses such as dendritic cell activation, B cell maturation, and T cell mediated tumor regression.